The toxic group had significantly lower concentrations of total proteins in the lens homogenate ( 0
The toxic group had significantly lower concentrations of total proteins in the lens homogenate ( 0.01) compared with a normal group [Table 2]. in unfavorable control. Conclusion: The results suggest that LCE can delay the onset and/or prevent the progression of cataract which can be attributed to the presence of adequate phenolics, flavonoids, and Vitamin A and its high nutritional value. This preliminary study can be further synergized by testing LCE against other and models Gonadorelin acetate of cataract. (Sponge gourd) belonging to family is widely used Gonadorelin acetate across the globe as a vegetable. Roem fruit extract (LCE) has been found to be an excellent antidiabetic and antioxidant.[1,2] Oxidative stress has been identified as an initiating factor in the development of cataract. It is a complex disease, characterized by opacification of lenses leading to blindness. Intraocular lens implantation is the most effective method to treat cataract, though rare, but it involves risks such as irreversible loss of vision, retinal detachment, and endophthalmitis.[4,5] Reduction of oxidative stress is considered as one of the targeting strategies for prevention or treatment of cataract. Hence, the present study was designed to assess the anticataract potential of LCE in H2O2 induced cataract in isolated goat lens through determination of lens morphology and estimation of some biochemical parameters such as superoxide dismutase (SOD), reduced glutathione (GSH), total protein content (TPC), and malondialdehyde (MDA) content in order to further potentiate a substantial preliminary correlation between antioxidant and anticataract activity in context with LCE. Materials and Methods Herb Extract and Other MaterialsLCE (Batch No. HNLC110850) was obtained from Herbo Nutra? New Delhi along with the certificate of analysis which stated that this extract complies with all the morphological specification of color, odor, taste, loss on drying, ash value, and microbial load of yeast, mold and by total plate count method. Preliminary phytochemical tests such as carbohydrates, starch, gums and mucilages, proteins and amino acids, fixed oils and fats, alkaloids, glycosides and flavonoids were performed, and the results were in conformity with the previously reported literature. LCE was extracted by the cold maceration method and concentrated by vacuum distillation to reduce the volume to 1/10. Hydrogen peroxide was purchased from Loba Chemie (Mumbai, India). Streptomycin and penicillin were obtained from Hindustan Antibiotics Ltd., (Pune, India). Marketed formulation (Catalin eye drops) containing pirenoxine which is a preparation of 1-hydroxy-5-oxo-5H-pyrido (3,2-)-phenoxazine-3-carboxylic acid, a compound having a chemical structure similar to xanthommatin, an eye pigment of the insect, with a pyridophenoxazone nucleus dissolved in 0.02% methylparaben, 0.01% propylparaben, and 0.0001% thimerosal as preservatives, available for treatment and prevention of cataract, was purchased from a medical Gonadorelin acetate store in Lucknow, India. Hydrogen peroxide was procured from LobaChemie, Navi Mumbai, India. All the chemicals used during the study were of analytical grade. Preparation of Lens CultureA total of 72 lenses, isolated from a group of 6C8 years old goats through extracapsular extraction, were used for the study. Age of goats was determined using teething method. These lenses were further divided into 9 groups containing 8 lenses each. Freshly extracted transparent lenses were incubated in tyrode physiological salt solution (PSS) containing sodium bicarbonate (0.9 g/ml), streptomycin (100 g/ml) and penicillin (100 IU/ml) at 37C in an incubator with 95% air and 5% CO2. The lenses were incubated initially for 2 h to discard any lens that had opacified due to damage during the extraction procedure. 1 ml of Rabbit Polyclonal to NFE2L3 H2O2 (0.5 mM) was used as cataract inducer. The quantity of catalin used was 1 ml. LCE was added in varying concentration of 5, 10, 15, 20, 25, and 30 g/ml of lens culture, respectively. Control Group Normal control: Lens + PSS + antibiotic solution Negative control: Lens + PSS + antibiotic solution + H2O2 solution Positive control: Lens + PSS + antibiotic solution + H2O2 solution + catalin. Experimental Group Lens + PSS + antibiotic solution + H2O2 solution + LCE solution (varying concentrations of 5, 10, 15, 20, 25, 30 g/ml of lens culture) In order to measure the degree of opacity of lenses, photographic evaluation was performed during the entire period of incubation at 0, 6th, 24th, 48th, and 72nd h. A grade of opacity was adopted which was based on the lens opacities classification system III. Grading of Opacity 0: Transparency 1: Slight cortical opacity 2: Diffuse cortical opacity 3: Dense nuclear cataract. Total Phenolic Content of Luffa cylindrica Roem Fruit ExtractTotal phenol content was estimated in APE by Folin-Ciocalteu’s reagent (FCR) based assay. To the aliquot (50 l) taken from stock solution (1 mg/ml) of the extracts,.