MiR-454-3p suppressed and targeted the expression of FRMD6, and linc00887 suppressed tumorigenesis of cervical cancer through activating the FRMD6-Hippo signaling pathway

MiR-454-3p suppressed and targeted the expression of FRMD6, and linc00887 suppressed tumorigenesis of cervical cancer through activating the FRMD6-Hippo signaling pathway. Conclusions Linc00887, sponging miR-454-3p, inhibited the development of cervical cancers by activating the FRMD6-Hippo signaling pathway. < 0.01 versus Scramble or Vector.(252K, doc) Acknowledgements Id prefer to express my honest thanks to those who've lent me personally hands throughout my composing this paper. Abbreviations lincRNALong intergenic noncoding RNAHPVHuman papillomavirusCINCervical intraepithelial neoplasiaGAS5Development arrest particular 5miRNAsMicroRNAsFRMD6FERM domain containing protein 6TIMP-1/2Tissue Inhibitor Of Matrix Metalloprotease-1/2MMP-9/2Matrix TLX1 metalloproteinase-9/2 Authors contributions JW and PL conceived and designed the tests. human regular (N?=?30), cervical cancers tissue (N?=?30), individual normal cervical epithelial cells (Ect1/E6E7) and cervical cancers cell lines (HeLa, C33A). After that, CCK-8 and Transwell assays were utilized to examine cell invasion and proliferation when linc00887 was overexpressed or knocked down. Furthermore, bioinformatics, luciferase reporter pull-down and gene PD176252 assays were utilized to predict and validate the partnership between linc00887 and miR-454-3p. Moreover, we discovered the appearance of miR-454-3p in Ect1/E6E7, C33A and HeLa cells when linc00887 was overexpressed or knocked straight down. Cell proliferation and invasion were measured when pcDNA-linc00887 and miR-454-3p were transfected by itself or jointly also. Next, miR-454-3p target gene was predicted and validated by luciferase and bioinformatics reporter gene assays. Gain- and loss-of-function tests had been performed in HeLa cells to judge the result of miR-454-3p or PD176252 linc00887 over the appearance of FERM domains containing proteins 6 (FRMD6) proteins and several essential protein in the FRMD6-Hippo signaling pathway. Outcomes Linc00887 was downregulated in cervical cancers tissues or individual cervical cancers cell lines (Hela, C33A) weighed against normal tissue or cell lines. Overexpression of linc00887 inhibited proliferation and invasion C33A and HeLa cells, while linc00887 knockdown acquired the opposite impact. Linc00887 destined with miR-454-3p, and overexpression of miR-454-3p rescued linc00887-induced inhibition invasion and proliferation of HeLa cells. MiR-454-3p suppressed and targeted the appearance of FRMD6, and linc00887 suppressed tumorigenesis of cervical cancers through activating the FRMD6-Hippo signaling pathway. Conclusions Linc00887, sponging miR-454-3p, inhibited the development of cervical cancers by activating the FRMD6-Hippo signaling pathway. < 0.01 versus PD176252 Vector or Scramble.(252K, doc) Acknowledgements Identification prefer to express my sincere because of all those who've lent me personally hands throughout my composing this paper. Abbreviations lincRNALong intergenic noncoding RNAHPVHuman papillomavirusCINCervical intraepithelial neoplasiaGAS5Development arrest particular 5miRNAsMicroRNAsFRMD6FERM domain filled with protein 6TIMP-1/2Tconcern Inhibitor Of Matrix Metalloprotease-1/2MMP-9/2Matrix metalloproteinase-9/2 Authors efforts PL and JW conceived and designed the tests. PL performed the tests. FC and LZ analyzed the info. LZ and PL wrote the manuscript. All authors read and accepted the manuscript. Financing None. Option of data and components The datasets utilized and/or analyzed through the present research can be found from the matching author on acceptable request. Ethics acceptance and consent to take part This research was accepted by the Moral Review Plank for Analysis in the Shaanxi Province Geriatric Medical center. Consent for publication All authors decided on the manuscript. Contending passions The authors declare that there surely is no conflict appealing about the publication of the paper. Footnotes Publisher's Take note Springer Nature continues to be neutral in regards to to jurisdictional promises in released maps and institutional affiliations. Contributor Details Pei Li, Email: moc.qq@6595113191. Jinsheng Wang, Email: moc.361@3201sjw. Lingran Zhi, Email: PD176252 moc.anis@ihznargnil. Fengmei Cai, Email: moc.621@3iac_iemgnef. Supplementary Details The online edition contains supplementary materials offered by 10.1186/s12935-020-01730-w..