Pim-1 The culture medium was RPMI-1640 medium supplemented with 5% heat-inactivated fetal calf serum, 4 mm l-glutamine, 25 mm HEPES (pH 72), 50 m 2-mercaptoethanol, 100 U/ml penicillin, 100 g/ml streptomycin and 025 g/ml amphotericin tuskonus March 2, 2025